Thermo Fisher Scientific GADD45 Polyclonal Antibody
GADD45 단백질을 인식하는 Rabbit Polyclonal 항체로 Western blot, IHC, ICC, Flow cytometry, ELISA에 사용 가능. Human, Mouse, Rat 반응성. Protein A 정제, 1 mg/mL 농도, -20°C 보관. 세포 스트레스 반응 및 G2/M 체크포인트 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:400 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Flow Cytometry (Flow) | 3 µg/test |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide derived from human GADD45 (amino acids 65–165) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01 M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
GADD45 alpha, beta, and gamma are evolutionarily conserved nuclear proteins functioning as stress sensors for cellular physiological and environmental damage. These proteins are essential for activation of the G2/M checkpoint induced by UV radiation or alkylating agents. GADD45-induced G2/M checkpoint regulation occurs through inactivation of Cdc2-cyclin B1 kinase. GADD45 forms a complex with p21 and Cdc2, serving as a core for interactions with other cell cycle regulators.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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