Thermo Fisher Scientific Rhotekin Polyclonal Antibody
Rhotekin 단백질을 인식하는 Rabbit Polyclonal 항체로 Western blot, IHC, ICC/IF에 사용 가능. Human에 반응하며, 항원 친화 크로마토그래피로 정제됨. PBS/glycerol 완충액에 보존되어 장기 안정성 우수. 연구용으로 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:500 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human Rhotekin. Recombinant protein control fragment (Product # RP-92032) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2646543 |
Product Specific Information
Immunogen sequence:
MTQPTASGTL RVQQAGEMQN WAQVHGVLKG TNLFCYRQPE DADTGEEPLL TIAVNKETRV RAGELDQALG RPFTLSISNQ YGDDEVTHTL QTESREALQS WMEALWQLFF DMSQWKQCC
Highest antigen sequence identity to orthologs:
- Mouse: 81%
- Rat: 80%
Target Information
This gene encodes a scaffold protein that interacts with GTP-bound Rho proteins. Binding of this protein inhibits the GTPase activity of Rho proteins. It may interfere with the conversion of active, GTP-bound Rho to the inactive GDP-bound form by RhoGAP.
Rho proteins regulate many important cellular processes, including cytokinesis, transcription, smooth muscle contraction, cell growth, and transformation. Dysregulation of the Rho signal transduction pathway has been implicated in many forms of cancer. Alternative splicing results in multiple transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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