Thermo Fisher Scientific IL-5 Polyclonal Antibody
Rat IL-5에 특이적인 Goat Polyclonal Antibody로, WB, ICC/IF, Neutralization 등 다양한 응용에 적합. 항원 친화 크로마토그래피로 정제되었으며, 보존제 무첨가 PBS 기반. 높은 특이성과 낮은 엔도톡신 수준으로 연구용에 적합.
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Thermo Fisher Scientific IL-5 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1 µg/mL |
| Immunocytochemistry (ICC/IF) | 5–15 µg/mL |
| Neutralization (Neu) | 2–6 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Rat |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Sf21-derived recombinant rat IL-5 (Met20–Val132) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No Preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2608059 |
Product Specific Information
- In direct ELISA, approximately 50% cross-reactivity with recombinant mouse IL-5 and 5% with recombinant human IL-5 is observed.
- Reconstitute at 0.2 mg/mL in sterile PBS.
- Endotoxin level: <0.10 EU per 1 µg antibody (LAL method).
Target Information
IL-5 is a cytokine that functions as a growth and differentiation factor for B cells and eosinophils. It regulates eosinopoiesis, eosinophil maturation, and activation. Elevated IL-5 levels are associated with asthma and hypereosinophilic syndromes. The IL-5 receptor is a heterodimer sharing its beta subunit with receptors for IL-3 and GM-CSF. IL-5, IL-4, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q31, coordinately regulated by long-range elements.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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(이미지 파일: PA5-47742_IL-5_Q08125-1_Goat.svg, PA5-47742_IL-5_Q08125-1_Goat_PDP.jpeg)
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