Thermo Fisher Scientific CellSensor DHFR/E2F-bla NIH3T3 Cell Line

Thermo Fisher Scientific CellSensor™ DHFR/E2F-bla NIH3T3 Cell Line

The CellSensor™ DHFR(E2F)-bla NIH3T3 Cell Line contains a beta-lactamase reporter gene under control of the E2F/DP1 binding sequence from the DHFR gene promoter, stably integrated into NIH 3T3 cells.
This cell line is a clonal population isolated by flow cytometry in response to 10% newborn bovine serum.

제품 설명

• E2F/DP1 결합 서열에 의해 조절되는 베타-락타마제 리포터 유전자를 포함
• 다양한 조건(DMSO 농도, 세포 수, 자극 시간, 기질 적재 시간)에서 성능 검증
• 신생 송아지 혈청을 사용한 Z-factor 검증 완료
• 대체 자극 조건에서도 데이터 확보
• G1/S 세포주기 체크포인트 연구에 적합

세포주기 조절 관련 배경

The G1/S cell-cycle checkpoint controls the transition from the first gap phase (G1) to the DNA synthesis phase (S).
Two key kinase complexes, CDK4/6-cyclin D and CDK2-cyclin E, along with the Rb-E2F transcription complex, regulate this checkpoint.

During G1, the Rb-HDAC repressor complex binds to E2F-DP1 transcription factors, inhibiting downstream transcription.
Phosphorylation of Rb by CDK4/6 and CDK2 dissociates the repressor complex, allowing transcription of S-phase genes essential for DNA replication and G1-to-S transition.

Various stimuli influence this checkpoint, including:

• TGFβ
• DNA damage
• Contact inhibition
• Replicative senescence
• Growth factor withdrawal

Academic and non-profit customers, please inquire for special pricing.

제품 사양