Thermo Fisher Scientific POLR2B Polyclonal Antibody
Thermo Fisher Scientific의 POLR2B 폴리클로날 항체는 인간, 생쥐, 랫드 시료에 반응하며 Western blot, IHC, ELISA에 적합합니다. 고순도 친화 크로마토그래피 정제, 액상 형태, 안정적인 PBS/glycerol 버퍼로 보관됩니다. 연구용으로 RNA polymerase II의 β 서브유닛 검출에 사용됩니다.
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Thermo Fisher Scientific POLR2B Polyclonal Antibody
Applications and Tested Dilutions
- Western Blot (WB): 1:500–1:2,000
- Immunohistochemistry (Paraffin) (IHC-P): 1:50–1:200
- ELISA: 1 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing amino acids 1–260 of human POLR2B (NP_000929.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.62 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2901664 |
Product Specific Information
- Positive Samples: SH-SY5Y, A375, HepG2, BT-474, Mouse brain, Mouse spleen, Mouse ovary, Rat ovary
- Immunogen Sequence:
MYDADEDMQY DEDDDEITPD LWQEACWIVI SSYFDEKGLV RQQLDSFDEF IQMSVQRIVE DAPPIDLQAE AQHASGEVEE PPRYLLKFEQ IYLSKPTHWE RDGAPSPMMP NEARLRNLTY SAPLYVDITK TVIKEGEEQL QTQHQKTFIG KIPIMLRSTY CLLNGLTDRD LCELNECPLD PGGYFIINGS EKVLIAQEKM ATNTVYVFAK KDSKYAYTGE CRSCLENSSR PTSTIWVSML ARGGQGAKKS AIGQRIVATL
Target Information
This gene encodes the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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