Invitrogen™

pMIB/V5-His Vector Kit

pMIB/V5-His is a 3.6 kb vector designed for constitutive expression and secretion of recombinant proteins from Sf9, Sf21, and High자세히 알아보기

pMIB/V5-His is a 3.6 kb vector designed for constitutive expression and secretion of recombinant proteins from Sf9, Sf21, and High Five™ insect cells. Secretion of proteins into serum-free medium simplifies purification, making it easier to harvest protein from cultured cells. pMIB/V5-His has several features to facilitate expression of recombinant proteins in insect cells including:

• The _OpIE_2 promoter for constitutive expression
• The honeybee melittin (HBM) secretion signal to allow secreted expression of your protein
• The Blasticidin resistance gene for rapid selection of stably transfected cell lines in two weeks
• C-terminal V5-epitope and polyhistidine tag for detection with Invitrogens Anti-V5 antibody and purification with nickel-chelating resin

사양

프로모터OplE2

제품 유형Insect Cell Expression Vector

단백질 태그 위치(유전자에 대해)C-terminal

단백질 태그His Tag (6x), V5 Epitope Tag

클로닝 방법Restriction Enzyme/MCS

수량1 kit

벡터pMIB

제품라인InsectSelect™

Unit Size1 kit