Thermo Fisher Scientific MCM4 Polyclonal Antibody
Rabbit 폴리클로날 항체로, 인간 MCM4 단백질을 인식합니다. Western blot 및 면역세포염색(ICC/IF)에 적합하며, 항원 친화 크로마토그래피로 정제되었습니다. PBS 기반 완충액에 40% 글리세롤 포함, 4°C 단기 보관 또는 -20°C 장기 보관 권장.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant Human MCM4. Recombinant protein control fragment (Product #RP-104624) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2662792 |
Product Specific Information
Immunogen sequence:
SSPPQMHSSAI PLDFDVSSPL TYGTPSSRVE GTPRSGVRGT PVRQRPDLGS AQKGLQVDLQ SDGAAAEDIV ASEQSLGQKL VIWGTDVNVA ACKENFQRFL QRFIDPLAKE EENVG
Highest antigen sequence identity to the following orthologs:
- Mouse: 95%
- Rat: 93%
Target Information
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) essential for the initiation of eukaryotic genome replication. The hexameric MCM complex is a key component of the pre-replication complex (pre_RC) and may be involved in replication fork formation and recruitment of other DNA replication-related proteins. The MCM complex (including MCM2, 6, and 7) possesses DNA helicase activity and may act as a DNA unwinding enzyme. Phosphorylation by CDC2 kinase reduces helicase activity and chromatin binding. This gene is located on chromosome 8, adjacent to PRKDC/DNA-PK, a DNA-activated protein kinase involved in double-strand break repair. Alternatively spliced transcript variants encoding the same protein have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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