Thermo Fisher Scientific Calpain 5 Polyclonal Antibody
Calpain 5 단백질을 인식하는 Rabbit Polyclonal 항체로 Western blot 및 Immunocytochemistry에 적합합니다. 인간에 반응하며, 농도 0.47 mg/mL의 액상 형태로 제공됩니다. 항원 친화 크로마토그래피로 정제되었으며, 단기 4°C, 장기 -20°C 보관이 권장됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 1–381 of Calpain-5 (Uniprot ID: O15484) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.47 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.01% thimerosal, 0.025% ProClin 300 |
| Storage conditions | Store at 4°C short term. For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_11157867 |
Product Specific Information
- Recommended positive controls: 293T, A431, H1299, HeLaS3
- Predicted reactivity: Mouse (93%), Rat (92%), Xenopus laevis (81%), Bovine (95%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Calpains are calcium-dependent cysteine proteases involved in signal transduction in various cellular processes. A functional calpain protein consists of an invariant small subunit and one of a family of large subunits. CAPN5 is one of the large subunits. Unlike some calpains, CAPN5 and CAPN6 lack a calmodulin-like domain IV. Due to its similarity to the Caenorhabditis elegans sex determination gene tra-3, CAPN5 is also referred to as HTRA3.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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