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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Takara · Takara AccIII (BspMII) restriction enzyme
AccIII (BspMII) Restriction Enzyme
Recognition Site
T | CCGG A
A GGCC | T
Methylation Sensitivity
This enzyme is capable of cleaving TC5mCGGA, but its rate is about 1/75.
Enzyme activity is affected by dam methylase, depending on the sequence following the recognition site.
Reaction Conditions
- Reaction temperature: 60°C
Product Information
| 항목 | 내용 |
|---|---|
| Source | Acinetobacter calcoaceticus |
| Concentration | 1–5 units/μl |
| Supplied buffer | Basal |
| Substrate for unit definition | Lambda DNA |
| Cleavage of pBR322 | Commercially available pBR322 is substantially undigested because adenine residues in the recognition site (TCCGGA) are methylated by dam methylase |
| Quality testing | Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting, and pKF3 cloning |
Additional Product Information
Please refer to the product’s Certificate of Analysis for details about storage conditions, product components, and technical specifications.
The Kit Components List is available under the Documents tab.
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FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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