
Takara AccIII (BspMII) restriction enzyme
AccIII(BspMII)는 Acinetobacter calcoaceticus 유래 제한효소로, TC5mCGGA 절단이 가능하며 dam 메틸화에 의해 활성 영향을 받습니다. 60°C에서 반응하며, lambda DNA를 기준으로 단위 정의가 이루어집니다. 품질 검증을 거친 고신뢰 연구용 효소입니다.
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AccIII (BspMII) Restriction Enzyme
Recognition Site
T | CCGG A
A GGCC | T
Methylation Sensitivity
This enzyme is capable of cleaving TC5mCGGA, but its rate is about 1/75.
Enzyme activity is affected by dam methylase, depending on the sequence following the recognition site.
Reaction Conditions
- Reaction temperature: 60°C
Product Information
| 항목 | 내용 |
|---|---|
| Source | Acinetobacter calcoaceticus |
| Concentration | 1–5 units/μl |
| Supplied buffer | Basal |
| Substrate for unit definition | Lambda DNA |
| Cleavage of pBR322 | Commercially available pBR322 is substantially undigested because adenine residues in the recognition site (TCCGGA) are methylated by dam methylase |
| Quality testing | Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting, and pKF3 cloning |
Additional Product Information
Please refer to the product’s Certificate of Analysis for details about storage conditions, product components, and technical specifications.
The Kit Components List is available under the Documents tab.
Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566
Asia Pacific: +1.650.919.7300
Europe: +33.(0)1.3904.6880
Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
© 2023 Takara Bio Inc. All Rights Reserved.
All trademarks are the property of Takara Bio Inc. or its affiliates.
Additional product, intellectual property, and restricted use information is available at takarabio.com.
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