Takara AccIII (BspMII) restriction enzyme

AccIII (BspMII) restriction site:

T | CCGG A
A GGCC | T

Effect of DNA methylation: This enzyme is capable of cleaving TC5mCGGA, but its rate is about 1/75. Otherwise, enzyme activity is affected by dam methylase

This enzyme is capable of cleaving TC5mCGGA, but its rate is about 1/75. Otherwise, enzyme activity is affected by dam methylase depending on the sequence following the recognition site.

Reaction temperature: 60°C

More Information

• Source: Acinetobacter calcoaceticus
• Concentration: 1–5 units/μl
• Supplied buffer: basal
• Substrate for unit definition: lambda DNA
• Cleavage of pBR322: commercially available pBR322 is substantially undigested because the adenine residues in the recognition site (TCCGGA) are methylated by dam methylase
• Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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