Invitrogen™

5-(3-Aminoallyl)-dUTP (50 mM)

When incorporated into DNA, Ambion 5-(3-aminoallyl)-dUTP provides a reactive group for addition of other chemical groups. The modified dUTP is자세히 알아보기

When incorporated into DNA, Ambion 5-(3-aminoallyl)-dUTP provides a reactive group for addition of other chemical groups. The modified dUTP is readily incorporated by reverse transcriptase. The aminoallyl modification enables downstream reaction with amine-reactive compounds such as N-hydroxysuccinimide (NHS) esters; thus aminoallyl-modified cDNA can be labeled with any amine-reactive group moiety.

Use of this modified nucleotide
Use 5-(3-aminoallyl)-dUTP as recommended in your protocol for reverse transcription. Substitution of 30–80% of the dTTP with 5-(3-aminoallyl)-dUTP may be useful, but the exact proportion will depend on experimental goals.

사양

형태Liquid

라벨 또는 염료Aminoallyl

제품라인Ambion™

제품 유형Nucleotides

정제 방법HPLC

수량50 μL

배송 조건Dry Ice

용도(애플리케이션)Nucleic Acid Labeling

농도50 mM

Unit SizeEach