Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (eBioJ105 (J105)), Biotin, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• APC
• APC-eFluor 780
• Brilliant UV 615
• Brilliant UV 661
• Brilliant UV 737
• Brilliant UV 805
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 650
• Brilliant Violet 711
• Brilliant Violet 786
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Super Bright 780
• Request custom conjugation

Product Specific Information

The J105 monoclonal antibody reacts with the human PD-1 (programmed death-1), a 55 kDa member of the CD28 immunoglobulin superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease. PD-1 is expressed predominantly on activated T and B lymphocytes. Two ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC), have been identified as PD-1 ligands, both showing overlapping functions and expression on normal and activated antigen-presenting cells.

Costaining experiments suggest that eBioJ105 recognizes a different epitope than MIH4 (Product #11-9969).

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of stimulated normal human peripheral blood cells. Recommended ≤0.5 µg per test (100 µL final volume, 10⁵–10⁸ cells/test). Optimize titration for best performance.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Cell-mediated immune responses are initiated by T lymphocytes stimulated by peptides bound to MHC molecules on antigen-presenting cells (APC). Activated T cells express PD-1 (PDCD-1), which mediates inhibitory signals upon binding to its ligands PD-L1 and PD-L2. These interactions downregulate immune activation in the absence of danger signals such as LPS. PD-1 knockout mice exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.