Thermo Fisher Scientific Fumarase Polyclonal Antibody
Fumarase 단백질을 인식하는 Rabbit Polyclonal 항체로 Western blot, IHC, ICC 등 다양한 응용에 적합. Human, Mouse, Rat에 반응하며 항원 친화 크로마토그래피로 정제됨. 4°C 또는 -20°C에서 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:10,000 | View 1 publication |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | – |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | – |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to amino acids 89–365 of Fumarate hydratase (Uniprot ID: P07954) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1M Tris glycine, pH 7, with 10% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term; for long-term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_11156640 |
Product Specific Information
- Recommended positive controls: 293T, A431, mouse liver, rat liver
- Predicted reactivity: Rat (96%), Zebrafish (86%), Pig (97%), Chicken (89%), Sheep (97%), Chimpanzee (99%), Bovine (96%)
- Store product as a concentrated solution. Centrifuge briefly before opening the vial.
Target Information
The fumarase protein is an enzymatic component of the tricarboxylic acid (Krebs) cycle, catalyzing the conversion of fumarate to L-malate. It exists in both cytosolic and mitochondrial forms, differing by the N-terminal extension. The mitochondrial form is processed to match the cytosolic version. This enzyme functions as a homotetramer and mutations in its gene can cause fumarase deficiency, leading to progressive encephalopathy.
⚠ WARNING: This product can expose you to chemicals including mercury, known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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