Thermo Fisher Scientific HSPA4 Polyclonal Antibody
HSPA4 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체입니다. Western blot, IHC, ICC/IF에 사용 가능하며 다양한 종에 반응합니다. 항원 친화 크로마토그래피로 정제되었으며, 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 75–374 of Human HSP70 4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | 0.1M Tris glycine, pH 7, with 10% glycerol |
| Contains | 0.01% thimerosal |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2546960 |
Product Specific Information
- Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, U87-MG, SK-N-SH, IMR32, SK-N-AS
- Predicted reactivity: Mouse (97%), Rat (96%), Xenopus laevis (87%), Dog (98%), Bovine (98%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The HSP70 family includes four highly conserved proteins: HSP70, HSC70, GRP75, and GRP78.
HSP70 expression is strongly induced in response to heat stress. HSP70 and HSC70 play key roles in cytosolic, endoplasmic reticulum, and mitochondrial import machinery, and are found in both the cytosol and nucleus of mammalian cells.
GRP78 is localized in the endoplasmic reticulum, involved in folding and translocation of nascent peptide chains.
GRP75 is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptides of both nuclear and mitochondrial origin.
Members of the HSP70 family act as force-generating motors, relying on ATP hydrolysis for their activity.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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