Thermo Fisher Scientific CRM1 Polyclonal Antibody
CRM1(XPO1) 단백질을 인식하는 Rabbit Polyclonal 항체로, IHC(P) 및 Flow Cytometry에 사용 가능. Human에 반응하며, sequence homology 기반으로 rat에도 예측 반응. PBS buffer에 보관되며, 장기 보관 시 -20°C에서 유지.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 |
| Flow Cytometry (Flow) | 1:10–1:50 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide between 817–846 amino acids from the C-terminal region of human XPO1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Ammonium sulfate precipitation, Size-exclusion - Dialysis |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2215454 |
Product Specific Information
This antibody is predicted to react with rat based on sequence homology.
Target Information
Exportin-1 (XPO1) mediates the nuclear export of cellular proteins bearing a leucine-rich nuclear export signal (NES) and RNAs. In the nucleus, in association with RANBP3, XPO1 binds cooperatively to the NES on its target protein and to the GTPase RAN in its active GTP-bound form (Ran-GTP). Docking of this complex to the nuclear pore complex is mediated through binding to nucleoproteins. Upon transit of a nuclear complex into the cytoplasm, disassembly of the complex and hydrolysis of Ran-GTP to Ran-GDP cause release of the cargo from the export receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP and GDP bound forms of Ran between the cytoplasm and nucleus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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