Thermo Fisher Scientific HIF1A Monoclonal Antibody (H1alpha67), DyLight 550

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 7 publications 7 publications

Immunohistochemistry (IHC)

-

View 19 publications 19 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

-

Immunohistochemistry (Frozen) (IHC (F))

1:100

-

Immunocytochemistry (ICC/IF)

Assay-Dependent

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-Dependent

-

ELISA (ELISA)

Assay-Dependent

-

Gel Shift (GS)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Xenopus, Sheep, Pig, Rabbit, Rat, Bovine, Dog, Cat, Ferret, Human, Mouse, Non-human primate

Published species

Human, Mouse, Rabbit, Rat

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

H1alpha67

Immunogen

Fusion protein containing amino acids 432-528 of human HIF-1alpha. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HIF1A,uniProtId:Q16665-1,ncbiNodeId:9606,antigenRange:432-528,antigenLength:826,antigenImageFileName:MA5-16010_HIF1A_Q16665-1_House_mouse.svg,antigenImageFileNamePDP:MA5-16010_HIF1A_Q16665-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

DyLight™ 550 DyLight™ 550 DyLight™ 550

View additional formats

• Unconjugated
• Biotin
• DyLight 488
• DyLight 650
• Request custom conjugation

Excitation/Emission Max

556/570 nm View spectra

Form

Liquid

Concentration

0.68 mg/mL

Purification

Protein G

Storage buffer

50mM sodium borate

Contains

0.05% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11157856

Product Specific Information

In Western blot, multiple bands may be seen at 100-120 kDa representing post-translational modification of HIF-1 alpha.

For WB, testing on nuclear extracts is recommended.

Suggested positive control: Cos-7 nuclear extracts.

Target Information

HIF1-alpha (HIF1A) is a subunit of HIF1, which is a transcription factor found in mammalian cells cultured under reduced oxygen tension. HIF-1 is a heterodimer consisting of an alpha and beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of transcription factors. HIF1 functions as a transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, HIF-1 activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF1-alpha regulates hypoxia-mediated apoptosis, cell proliferation and tumor angiogenesis. Hypoxia which induces p53 protein accumulation, directly interacts with HIF1-alpha and reduces hypoxia-induced expression of HIF1-alpha by promoting MDM2-mediated ubiquitination and proteasomal degradation under hypoxic conditions. Recent studies suggest that induction of NOX4 by HIF1-alpha contributes to maintain ROS levels after hypoxia and hypoxia-induced proliferation. In humans, it is located on the q arm of chromosome 14. The C-terminal of HIF1A binds to p300. p300/CBP-HIF complexes participate in the induction of hypoxia-responsive genes, including VEGF. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.