Thermo Fisher Scientific MCP-4 (CCL13) Human ProcartaPlex Simplex Kit

Invitrogen™ MCP-4 (CCL13) Human ProcartaPlex™ Simplex Kit

The Human MCP-4 (CCL13) Simplex ProcartaPlex Kit measures MCP-4 (CCL13) protein and is designed to be combinable with other Simplex kits, allowing creation of custom multiplex panels utilizing Luminex xMAP technology for protein detection and quantitation.
When combining multiple Simplex kits (not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is required per assay plate regardless of plex size.

About ProcartaPlex Assays for the Luminex Platform

ProcartaPlex immunoassays are based on sandwich ELISA principles, using two highly specific antibodies binding to different epitopes of one protein to quantitate multiple protein targets simultaneously using a Luminex instrument.

• More results per sample: Measure multiple protein targets simultaneously in a single 25–50 μL sample
• Well-established Luminex technology: Highly referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 μL sample from plasma, serum, cell culture supernatants, and other bodily fluids.

In the ProcartaPlex assay, Luminex beads are internally dyed with precise proportions of red and infrared fluorophores to create spectrally unique signatures identifiable by Luminex xMAP detection systems (e.g., Luminex 200, FLEXMAP 3D, MAGPIX).
Similar to a sandwich ELISA, matched antibody pairs identify the protein of interest. Each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are detected with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE).
The conjugation of protein-specific antibodies to distinct beads enables multiplexed analysis in a single well.

The major difference between ProcartaPlex assay and ELISA is that the capture antibody in ProcartaPlex is conjugated to a bead rather than adsorbed to a microplate well, allowing reagents to remain free-floating.
For detection, instruments like Luminex 200 use two lasers: one to identify bead spectral signatures and another to quantify RPE fluorescence proportional to protein amount.
ProcartaPlex multiplex assays can profile more target proteins using significantly less sample within the same time required for a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, canine).
Visit thermofisher.com/procartaplex for a comprehensive list of protein targets.

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