Thermo Fisher Scientific AgriSeq HTS Library Kit, 9600 reactions

Applied Biosystems™ AgriSeq™ HTS Library Kit, 9600 Reactions

The Applied Biosystems AgriSeq HTS Library Kit is specifically designed for high-throughput preparation of amplicon libraries for targeted genotyping-by-sequencing (GBS) applications in agrigenomics.

Key Product Features

• Exceptional uniformity of multiplexed target amplification for robust, reproducible results
• Simplified workflow enabling high-throughput sample processing
• Convenient configuration including library normalization reagents and volumes suitable for automation

Excellent Data Quality

AgriSeq library preparation is optimized for targeted GBS panels ranging from 100–5000 amplicons.
Using efficient multiplex technology, AgriSeq amplification achieves:

• Uniformity: 87.2–99.3%
• Percent on-target reads: 85.7–99.7%
• Genotype call rates: 87.5–99.7%

An average of 98.2% uniformity was observed for a 3000-amplicon panel across 192 samples, meaning 98% of bases are covered by at least 0.2X of the average read depth. This even coverage reduces sequencing requirements and overall cost per sample.
Compared to competitor technology (TruSeq), AgriSeq demonstrates higher uniformity and multiplexing capability (reference).

High Throughput Sample Processing

The AgriSeq Library workflow supports both 96- and 384-well plate formats, requiring as little as 10 ng of DNA input per reaction.
Optimized for minimal sample handling and reagent usage, the workflow allows pooled sample processing with fewer pipetting steps—reducing error and simplifying automation setup on platforms such as Tecan or Hamilton.

Data analysis includes:

• Automated quality assessment
• Alignment and genotype calling
• Integrated Torrent Suite software and Variant Caller plug-in
  No need for additional bioinformatics expertise or third-party software.

Convenient Packaging for Automation

Available in large pack sizes (960 or 9600 reactions), the AgriSeq HTS Kit minimizes cost and packaging waste for high-throughput agrigenomics projects.
Includes reagents for both library preparation and normalization, with sufficient overage for automated workflows.
AgriSeq targeted GBS offers a cost-effective solution for generating high-throughput genotyping data to support breeding program advancement.

제품 사양

제품 이미지

(이미지 없음)