Thermo Fisher Scientific MHC Class I (H-2Db) Monoclonal Antibody (28-14-8), PE, eBioscience
마우스 MHC Class I (H-2Db)에 특이적인 단클론 항체로, PE 형광 표지되어 있습니다. Flow cytometry에 최적화되어 있으며, 0.5 µg/test로 사용 가능합니다. 28-14-8 클론으로 다양한 MHC 아형과 교차 반응합니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/test | View 14 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse, Rat |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 28-14-8 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_466124 |
Available Formats
- Unconjugated
- APC
- Biotin
- eFluor 450
- FITC
- Functional Grade
- NovaFluor Blue 510 / 585 / 610-70S / 660-120S / 690
- NovaFluor Red 710 / 755
- NovaFluor Yellow 570 / 660 / 730 / 755
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Request custom conjugation
Product Specific Information
The 28-14-8 monoclonal antibody reacts with mouse MHC class I, H-2D^b, and cross-reacts with H-2L^d, H-2D^q, and/or H-2L^q. Binding to the alpha 3 domain of H-2L^d is not dependent on beta2-microglobulin.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse splenocytes at ≤0.5 µg/test (100 µL final volume). Optimal titration recommended.
Excitation: 488–561 nm
Emission: 578 nm
Compatible Lasers: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
This protein is an MHC class I alloantigen involved in antigen presentation to CD3/TCR and CD8-expressing T cells.
HLA and MHC antibodies are key tools in immunopeptidomics, aiding in the identification and characterization of neoantigens via LC-MS/MS.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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배송/결제/교환/반품 안내
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