Thermo Fisher Scientific AMPK beta-2 Monoclonal Antibody (7A8C3)
AMPK beta-2 단백질을 인식하는 Mouse IgG2b 단일클론 항체로, 인간 시료에 반응합니다. Western blot, Flow cytometry, ELISA에 적합하며, 고순도 Protein G 정제 및 1 mg/mL 농도의 액상 형태입니다. 에너지 대사 관련 연구용으로 사용됩니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 7A8C3 |
| Immunogen | Purified recombinant fragment of human PRKAB2 (AA: 1–120) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2787486 |
Product Specific Information
MA5-31863 has been tested in indirect ELISA.
Target Information
AMPK Beta 2 is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit and non-catalytic beta and gamma subunits. It functions as an energy-sensing enzyme that monitors cellular energy status. Upon activation by metabolic stress, AMPK phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and HMG-CoA reductase (HMGCR), key enzymes regulating fatty acid and cholesterol biosynthesis. The beta-2 subunit may positively regulate AMPK activity and affect enzyme localization through myristoylation and phosphorylation. It may also serve as an adaptor molecule mediating AMPK complex assembly.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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