Thermo Fisher Scientific MIP-3 beta Monoclonal Antibody (54909)
인간 MIP-3 beta(CCL19)을 인식하는 마우스 단클론 항체로, Western blot, IHC, ICC, Flow Cytometry 등에 사용 가능. 단백질 A/G로 정제된 1.0 mg/mL 액상 제품이며, 보존제 무첨가. -20°C에서 보관하며 동결/해동 반복을 피해야 함.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | View 1 publication |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 8–25 µg/mL | - |
| Immunocytochemistry (ICC/IF) | 8–25 µg/mL | - |
| Flow Cytometry (Flow) | 0.25 µg per million cells | - |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 54909 |
| Immunogen | E. coli-derived recombinant human CCL19/MIP-3 beta (Gly22–Ser98) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | 600 mM Tris, 500 mM acetic acid |
| Contains | No Preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2605835 |
Target Information
CCL19, also known as MIP-3 beta (Macrophage Inflammatory Protein 3 beta), is a member of the CC subfamily of chemokines. It is closely related to CCL21, sharing 32% amino acid sequence homology.
CCL19 and CCL21 are mainly expressed by stromal cells in T cell-rich zones of lymph nodes and are key mediators of homeostatic trafficking of naive T cells and activated dendritic cells into secondary lymphoid organs.
They play essential roles in T cell priming, activation, and lymphocyte recruitment to inflamed tissues. Both signal through the G protein-coupled receptor CCR7, expressed on T cells and mature dendritic cells.
Although both ligands bind CCR7 with similar affinity, only CCL19 binding induces receptor desensitization and internalization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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