ELK Biotechnology MEF-2A/C rabbit pAb

제품명

MEF-2A/C rabbit pAb

제품 개요

Myocyte-specific enhancer factor 2A/C (MEF2A/C)는 세포 성장, 생존, 근육 및 신경 발달에 관여하는 전사 인자로, 본 항체는 인간 유래 합성 펩타이드로부터 제작된 rabbit polyclonal antibody입니다.

응용 분야

• WB (Western Blot)
• ELISA

권장 희석 배수

• WB: 1:1000–2000
• ELISA: 1:5000–20000

제품 사양

Background

Defects in MEF2A may cause autosomal dominant coronary artery disease 1 with myocardial infarction (ADCAD1) [MIM:608320].
MEF2A acts as a transcriptional activator binding specifically to the MEF2 element found in various muscle-specific genes and regulates growth factor- and stress-induced genes. It plays roles in skeletal and cardiac muscle development, neuronal differentiation, and survival.

Post-translational Modifications (PTM):

• Acetylation on Lys-403 activates transcriptional activity; hyperacetylation leads to cardiac myocyte growth and heart failure.
• Phosphorylation on Ser-408 promotes sumoylation at Lys-403, preventing acetylation.
• Dephosphorylation by PPP3CA switches sumoylation to acetylation, inhibiting dendrite differentiation.
• Phosphorylation on Thr-312/319 activates transcription via p38 MAPK signaling.
• Caspase-mediated cleavage under neurotoxicity leads to neuronal apoptosis.
• Sumoylation on Lys-403 represses transcriptional activity, facilitated by PIAS1 and SUMO1/2/3.

Similarity and Structure:

• Belongs to the MEF2 family.
• Contains 1 MADS-box domain and 1 Mef2-type DNA-binding domain.

Protein Interactions:

• Forms homo-/heterodimers with MEF2D.
• Interacts with HDAC4, HDAC7, HDAC9, PIAS1, and MAPK7.

Tissue Specificity:

• Isoforms MEF2 and MEFA: skeletal and cardiac muscle, brain.
• Isoforms RSRFC4 and RSRFC9: expressed in all tissues examined.