ELK Biotechnology Phospho PKC θ (Y90) rabbit pAb

제품명

Phospho PKC θ (Y90) rabbit pAb

제품 개요

Phospho PKC θ (Y90) rabbit pAb는 인간 Phospho PKC θ (Y90)에서 유래한 합성 펩타이드를 면역원으로 제작된 다클론 항체입니다. T세포 수용체(TCR) 자극에 반응하는 PKC θ의 인산화 상태를 검출하는 데 사용됩니다.

제품 정보

Background

• Catalytic activity: ATP + a protein = ADP + a phosphoprotein
• Cofactor: Magnesium
• Domain: The C1 domain (C1A, C1B) acts as a diacylglycerol sensor, while the C2 domain is non-calcium binding.
• Enzyme regulation: Phosphorylation at Thr-538, Ser-676, and Ser-695 is required for full activation.
• Function:
  • Activated by diacylglycerol, phosphorylates various cellular proteins.
  • Serves as a receptor for phorbol esters (tumor promoters).
  • Calcium-independent, phospholipid-dependent serine/threonine kinase.
  • Essential for T-cell receptor (TCR)-mediated activation and NF-κB signaling in mature T lymphocytes.
  • Required for interleukin-2 (IL-2) production.
• PTM: Autophosphorylation at Thr-219 is necessary for TCR targeting and function.
• Similarity:
  • Belongs to the protein kinase superfamily, AGC Ser/Thr protein kinase family, PKC subfamily.
  • Contains 1 AGC-kinase C-terminal domain, 1 C2 domain, 1 protein kinase domain, 2 phorbol-ester/DAG-type zinc fingers.
• Subunit: Interacts with TXNL2/PICOT.
• Tissue specificity: Expressed in skeletal muscle, megakaryoblastic cells, and platelets.