ELK Biotechnology TOP2A (Phospho Ser1213) rabbit pAb
TOP2A (Phospho Ser1213) rabbit polyclonal antibody for detection of phosphorylated TOP2A in human, rat, and mouse samples. Suitable for WB and ELISA applications. High specificity and sensitivity with long-term storage at -20°C.
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제품명
TOP2A (Phospho Ser1213) rabbit pAb
제품 정보
| 항목 | 내용 |
|---|---|
| Alternative Names | DNA topoisomerase 2-alpha (EC 5.99.1.3; DNA topoisomerase II, alpha isozyme) |
| Applications | WB; ELISA |
| Recommended Dilutions | WB 1:1000–2000, ELISA 1:5000–20000 |
| Immunogen | Synthesized peptide derived from human TOP2A (Phospho Ser1213) |
| Host | Rabbit |
| Storage | -20°C / 1 year |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1 mg/ml |
| Observed Band | 174 kD |
| GeneID (Human) | 7153 |
| Human Swiss-Prot No | P11388 |
| Cellular Localization | Cytoplasm; Nucleus (nucleoplasm, nucleolus) |
| Species Reactivity | Human; Rat; Mouse |
Background
TOP2A (DNA topoisomerase II alpha) is an enzyme that controls the topological states of DNA through ATP-dependent breakage, passage, and rejoining of double-stranded DNA. It is specifically inhibited by the intercalating agent amsacrine. Both eukaryotic topoisomerase I and II can relax negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils. Phosphorylation has no effect on catalytic activity. TOP2A generally localizes in the nucleoplasm and functions as a homodimer interacting with COPS5.
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