ELK Biotechnology SRY (Acetyl Lys136) rabbit pAb

제품명

SRY (Acetyl Lys136) rabbit pAb

대체 명칭

Sex-determining region Y protein (Testis-determining factor)

응용 분야

• Western Blot (WB)
• ELISA

권장 희석 배율

• WB: 1:1000–2000
• ELISA: 1:5000–20000

면역원

Synthesized peptide derived from human SRY (Acetyl Lys136)

저장 조건

-20°C, 1년 보관

항체 정보

세포 내 위치

Nucleus speckle, Cytoplasm, Nucleus.
Acetylation contributes to nuclear localization, while deacetylation by HDAC3 induces cytoplasmic delocalization (PubMed:15297880).
Colocalizes with SOX6 (PubMed:11818535), CAML (PubMed:15746192), ZNF208 isoform KRAB-O, and tyrosine hydroxylase (TH).
Nuclear import facilitated by XPO4 (PubMed:19349578).

배경 정보

• 질환 관련성:
  • Defects in SRY cause gonadal dysgenesis XY female type (GDXY) [MIM:306100], also known as Swyer syndrome.
  • Defects in SRY are also associated with true hermaphroditism [MIM:235600] and Turner syndrome.
• 기능:
  Transcriptional regulator controlling male development by directing Sertoli cell differentiation.
  Involved in promoter activation/repression and pre-mRNA splicing.
  Recognizes DNA consensus sequence 5'-[AT]AACAA[AT]-3'.
• 기타 정보:
  Human and mouse SRY HMG domains differ in DNA binding properties.
  Phosphorylation by PKA enhances DNA-binding and transcription repression.
  Acetylation at Lys-136 promotes nuclear localization and interaction with KPNB1.
  Deacetylated by HDAC3, poly-ADP-ribosylated by PARP1.
  Belongs to the SRY family and contains one HMG box DNA-binding domain.
• 단백질 상호작용:
  Interacts with CALM, EP300, HDAC3, KPNB1, ZNF208 isoform KRAB-O, PARP1, SLC9A3R2, and WT1.
  EP300 interaction modulates DNA-binding activity.
  KPNB1 interaction is sensitive to Ran-GTP dissociation.