Thermo Fisher Scientific Qtracker 585 Cell Labeling Kit

Invitrogen™ Qtracker™ 585 Cell Labeling Kit

The Qtracker™ 585 Cell Labeling Kit uses a custom targeting peptide to deliver yellow-fluorescent Qdot™ 585 nanocrystals into the cytoplasm of live cells. These kits are designed for loading cultured cells with highly fluorescent Qdot™ nanocrystals. Once inside, Qtracker™ labels provide intense, stable fluorescence that can be traced through several generations and are not transferred to adjacent cells.

Need a different emission spectrum or longer tracking? View other mammalian cell tracking products.

Key Attributes

• Qtracker™ 585 label has Ex/Em (405–545/585 nm)
• Designed for loading cells grown in culture with highly fluorescent Qdot™ nanocrystals
• Provides intense, stable fluorescence that can be traced through several generations
• Available in eight colors: 525 nm, 565 nm, 585 nm, 605 nm, 625 nm, 655 nm, 705 nm, 800 nm emission
• Excellent for long-term tracking or imaging studies of live cells (migration, motility, morphology, and other assays)

Product Description

Qtracker™ kits use a custom targeting peptide to deliver Qdot™ nanocrystals into the cytoplasm of live cells. Delivery is typically completed within 1 hour and is not cell-type specific. The Qdot™ nanocrystals are compatible with serum-sensitive cells and maintain intense fluorescence under various biological conditions (pH, temperature, metabolic changes). Autofluorescence can be minimized using Qtracker™ 655, 705, or 800 Kits.

Long-Lasting, Targeted Signal

Qtracker™ labels resist photobleaching and degradation. They remain in cytoplasmic vesicles and are inherited by daughter cells for at least six generations. Fluorescence can persist up to a week post-labeling, making these kits ideal for studies of cell motility, migration, differentiation, and morphology. The labels do not leak or transfer between adjacent cells.

Monitoring Platforms

Labeled cells can be monitored using:

• Flow cytometry
• Fluorescence or confocal microscopy
• Fluorescence microplate readers
• High-content imaging systems

Minimal Impact on Live Cells

Cytotoxicity tests across multiple cell lines (CHO, HeLa, U-118, 3T3, HUVEC, Jurkat) show minimal impact on surface marker expression, proliferation, enzyme activity, and motility.

Applications

Qtracker™ labeled cells are useful for:

• Co-culture and heterotypic assembly studies
• Multilineage differentiation and trans-differentiation
• Embryonic and mesenchymal stem cell tracking
• Cell migration dynamics

Specifications