Thermo Fisher Scientific Adenylate Kinase 4 Polyclonal Antibody
Rabbit polyclonal antibody against Adenylate Kinase 4, validated for WB, IHC(P), and ICC/IF. Reacts with human, predicted cross-reactivity with mouse, rat, and bovine. Supplied as liquid, 1 mg/mL, antigen affinity purified, for research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1–209 (P27144) of Adenylate Kinase 3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1M Tris glycine, pH 7, with 10% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_11156584 |
Product Specific Information
- Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Raji
- Predicted reactivity: Mouse (89%), Rat (88%), Bovine (92%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene encodes a member of the adenylate kinase family of enzymes localized to the mitochondrial matrix. Adenylate kinases regulate adenine and guanine nucleotide composition by catalyzing the reversible transfer of phosphate groups among nucleotides. Five isozymes have been identified in vertebrates, showing tissue-specific and developmentally regulated expression. A pseudogene is located on chromosome 17, and three transcript variants encoding the same protein have been identified on chromosome 1 (NM_013410, NM_203464, and NM_001005353).
Safety Information
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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