Thermo Fisher Scientific FAS Monoclonal Antibody (H.831.6)
FAS (CD95) 단백질을 인식하는 Rabbit monoclonal antibody (Clone H.831.6). Western blot, IHC, ICC 등 다양한 응용에 적합. Human 및 Rat 시료 반응성. 고순도 친화 크로마토그래피 정제, -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 1 publication |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:250–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Rat |
| Published Species | Human, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | H.831.6 |
| Immunogen | Synthetic peptide corresponding to residues surrounding Ile262 within the intracellular region of human Fas protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 206 µg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.01M HEPES (pH 7.5), 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | <0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_11009620 |
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
FAS (Fas, CD95, APO-1) is a 46 kDa transmembrane glycoprotein functioning as a cell death receptor in the TNFR superfamily. It also exists in a soluble 26 kDa form. Activation of FAS triggers aggregation of FADD domains, forming the death-inducing signaling complex (DISC) and activating caspases, leading to apoptosis. FAS can also activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, contributing to proliferative signaling in fibroblasts and T cells. Multiple alternatively spliced variants exist, some lacking the transmembrane domain, which may regulate apoptosis negatively. The Fas/Fas ligand pathway is implicated in diseases such as AIDS, hepatitis, and cancer, and plays a role in antiviral immune responses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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