Thermo Fisher Scientific Histone H2A.X Polyclonal Antibody
Histone H2A.X 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 사용 가능. Human, Mouse, Rat에서 반응하며, 항원 친화 크로마토그래피로 정제됨. DNA 손상 연구 및 감마-H2AX 검출에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within Human Histone H2AX aa 1–50 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.4, with 0.2% BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage conditions | Store at 4°C (short term). For long-term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2901073 |
Product Specific Information
Positive controls include MCF-7 cell lysates, SHSY5Y, rat testicular tissue, human lung cancer tissue, human liver tissue, and mouse fallopian tube tissue.
Target Information
Histone H2A.X (H2AX) is a member of the histone H2A family, one of the four core histones forming the nucleosome core particle. DNA double-strand breaks (DSBs) induce phosphorylation of serine 139 at the C-terminus of H2AX, producing gamma-H2AX, a marker for DNA damage. This phosphorylation can be detected by Western blotting or immunofluorescence to assess DSB frequency. Phosphorylation is mediated by phosphatidylinositol 3-kinases such as ATM, DNA-PK, and ATR.
H2A.X consists of 143 amino acids and is synthesized in both G1 and S phases. Its mRNA exists in two forms: a 1600-base polyA form and a 575-base non-polyA form, corresponding to basal and replication-type histone mRNAs, respectively. The gene maps to human chromosome 11q23.2–q23.3.
Histone H2A.X plays a key role in chromatin structure and DNA repair, particularly through interaction with MDC1 at DNA damage sites.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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