Takara Bacterial alkaline phosphatase (E. coli)

More Information

Applications

• Phosphate group removal to reduce self-ligation and vector background
• Dephosphorylation of DNA 5` termini prior to kinase labeling reactions

Source

E. coli strain C75

Storage

–20°C

Note

Bacterial alkaline phosphatase (E. coli) is extremely stable and is not completely heat-inactivated; therefore, to inactivate the enzyme, reaction products need to be purified twice via phenol-chloroform extraction followed by ethanol precipitation.

Buffer

Supplied with 10X Reaction Buffer [500 mM Tris-HCl (pH 9.0), 10 mM MgCl2]

Unit definition

One unit is defined as the amount of enzyme required to generate 1 µmol/min of p-nitrophenol from p-nitrophenylphosphate at 25°C and pH 8.0.

Concentration

0.5 U/µl

Product citations

Reid, T. W. & Wilson, I. B. E. coli Alkaline Phosphatase. Enzym. 4, 373–415 (1971).

Takanami, M. Analysis of the 5-terminal nucleotide sequences of ribonucleic acids: I. The 5-termini of Escherichia coli ribosomal RNA. J. Mol. Biol. 23, 135–48 (1967).

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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