Takara Bacterial alkaline phosphatase (E. coli)

Takara Bacterial Alkaline Phosphatase (E. coli)

Applications

• Phosphate group removal to reduce self-ligation and vector background
• Dephosphorylation of DNA 5′ termini prior to kinase labeling reactions

Source

E. coli strain C75

Storage

–20°C

Note

Bacterial alkaline phosphatase (E. coli) is extremely stable and not completely heat-inactivated. To inactivate the enzyme, reaction products should be purified twice using phenol-chloroform extraction followed by ethanol precipitation.

Buffer

Supplied with 10X Reaction Buffer containing:

• 500 mM Tris-HCl (pH 9.0)
• 10 mM MgCl₂

Unit Definition

One unit is defined as the amount of enzyme required to generate 1 µmol/min of p-nitrophenol from p-nitrophenylphosphate at 25°C and pH 8.0.

Concentration

0.5 U/µl

Product Citations

• Reid, T. W. & Wilson, I. B. E. coli Alkaline Phosphatase. Enzym. 4, 373–415 (1971).
• Takanami, M. Analysis of the 5′-terminal nucleotide sequences of ribonucleic acids: I. The 5′-termini of Escherichia coli ribosomal RNA. J. Mol. Biol. 23, 135–48 (1967).

Additional Product Information

Please refer to the product’s Certificate of Analysis for detailed information about storage conditions, product components, and technical specifications. The Kit Components List is also available under the Documents tab.

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FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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Specifications