상품 옵션 정보 | ||||||
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카탈로그 번호 | 설명 | 상태 | 단위 | 가격 | 가격(VAT포함) | 수량 / 장바구니 / 찜 |
2120B | Takara 2120B Alkaline Phosphatase (E coli C75), 250 Units pk | 재고문의 | pk | 380,000원 | 418,000원 | |
2120A | Takara 2120A Alkaline Phosphatase (E coli C75), 50 Units pk | 재고문의 | pk | 85,000원 | 93,500원 |
More Information
Applications
- Phosphate group removal to reduce self-ligation and vector background
- Dephosphorylation of DNA 5` termini prior to kinase labeling reactions
Source
E. coli strain C75
Storage
–20°C
Note
Bacterial alkaline phosphatase (E. coli) is extremely stable and is not completely heat-inactivated; therefore, to inactivate the enzyme, reaction products need to be purified twice via phenol-chloroform extraction followed by ethanol precipitation.
Buffer
Supplied with 10X Reaction Buffer [500 mM Tris-HCl (pH 9.0), 10 mM MgCl2]
Unit definition
One unit is defined as the amount of enzyme required to generate 1 µmol/min of p-nitrophenol from p-nitrophenylphosphate at 25°C and pH 8.0.
Concentration
0.5 U/µl
Product citations
Reid, T. W. & Wilson, I. B. E. coli Alkaline Phosphatase. Enzym. 4, 373–415 (1971).
Takanami, M. Analysis of the 5-terminal nucleotide sequences of ribonucleic acids: I. The 5
-termini of Escherichia coli ribosomal RNA. J. Mol. Biol. 23, 135–48 (1967).
Additional product information
Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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