Thermo Fisher Scientific B-Myb Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Recommended positive control: HepG2
• Predicted reactivity:
  Cow: 100%; Dog: 100%; Guinea Pig: 85%; Horse: 100%; Rabbit: 92%; Rat: 78%; Zebrafish: 83%
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Nucleocytoplasmic transport, a signal- and energy-dependent process, occurs through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal requires the NLS import receptor, a heterodimer of importin alpha and beta subunits (karyopherins). Importin alpha binds the NLS-containing cargo in the cytoplasm, and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP-binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein, while importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential for translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.