Thermo Fisher Scientific PHGDH Polyclonal Antibody
PHGDH 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, IP 등 다양한 응용에 사용 가능. Human 및 Mouse 반응성. 항원 친화 정제 방식으로 제조되었으며, 액상 형태로 4°C에서 보관. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 1 to 50 of human Phosphoglycerate Dehydrogenase |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Phosphate/Tris citrate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt.
- For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
- Based on 100% sequence identity, this antibody is predicted to react with Bovine, Pig, Orangutan, and Chimpanzee.
Target Information
The PHGDH gene encodes the enzyme involved in the early steps of L-serine synthesis in animal cells.
L-serine is essential for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity.
Mutations in this gene have been associated with congenital microcephaly, psychomotor retardation, and other symptoms.
Multiple alternatively spliced transcript variants have been identified, though the full-length nature of most is unknown.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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