
Thermo Fisher Scientific Goat anti-Chicken IgY (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 555
Alexa Fluor Plus 555 형광으로 표지된 염소 유래 항-닭 IgY (H+L) 2차 항체로, 높은 특이성과 낮은 배경 신호를 제공. WB 및 ICC/IF 등 형광 이미징에 최적화. 교차흡착 처리로 비특이적 결합 최소화. 연구용으로만 사용.
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Thermo Fisher Scientific Goat anti-Chicken IgY (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 555
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.4 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chicken |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Purified Chicken IgY, Heavy and Light chains |
| Conjugate | Alexa Fluor™ Plus 555 |
| Excitation / Emission Max | 558 / 572 nm |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 6.5 |
| Contains | 0.016% Methylisothiazolone |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2762844 |
| Target | IgY |
| Cross Adsorption | Against chicken serum containing non-immunoglobulin chicken serum proteins |
| Antibody Form | Whole Antibody |
Product Specific Information
To minimize cross-reactivity, the goat anti-chicken IgY whole antibodies have been affinity-purified and cross-adsorbed against chicken serum containing non-immunoglobulin chicken serum proteins.
Cross-adsorption increases antibody specificity, resulting in higher sensitivity and less background staining.
The secondary antibody solution is passed through a column containing immobilized serum proteins from potentially cross-reactive species, capturing nonspecific antibodies and allowing highly specific ones to flow through.
Usage Tips:
- Centrifuge conjugate solution briefly before use and use only the supernatant to reduce nonspecific staining.
- Optimal antibody dilution should be determined empirically.
Specificity:
This antibody binds to heavy chains on chicken IgY and light chains on all chicken immunoglobulins. It does not bind non-immunoglobulin chicken serum proteins.
Target Information
Anti-Chicken secondary antibodies are affinity-purified with well-characterized specificity for chicken immunoglobulins.
They are useful for detection, sorting, or purification of chicken IgY targets.
Secondary antibodies enhance sensitivity through signal amplification and can be conjugated to various labels (e.g., HRP, AP, fluorescence).
Typically generated by immunizing host animals with pooled immunoglobulins from the target species, and further purified or modified for high specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Available Formats
- Alexa Fluor 633
- Alexa Fluor Plus 405
- Alexa Fluor Plus 488
- Alexa Fluor Plus 594
- Alexa Fluor Plus 647
- Alexa Fluor Plus 680
- Alexa Fluor Plus 800
- AP
- Biotin
- DyLight 350, 488, 550, 594, 650, 680, 755, 800
- TRITC
- Custom conjugation available upon request
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