Thermo Fisher Scientific HuC/HuD Monoclonal Antibody (16A11)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

View 15 publications 15 publications

Immunohistochemistry (IHC)

-

View 343 publications 343 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 24 publications 24 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

-

View 3 publications 3 publications

Immunohistochemistry (Frozen) (IHC (F))

5-20 µg/mL

View 43 publications 43 publications

Immunohistochemistry - Free Floating (IHC (Free))

-

View 6 publications 6 publications

Immunocytochemistry (ICC/IF)

5-20 µg/mL

View 67 publications 67 publications

Flow Cytometry (Flow)

-

View 1 publication 1 publication

ELISA (ELISA)

-

View 1 publication 1 publication

Functional Assay (Functional)

-

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

-

View 41 publications 41 publications

Product Specifications

Species Reactivity

Avian, Chicken, Human, Zebrafish

Published species

Amphibian, Avian, Cat, Chicken, Chimpanzee, Fish, Guinea pig, Horse, Human, Lizard, Mouse, Non-human primate, Pig, Rabbit, Rat, Reptile, Rhesus monkey, Rodent, Shark, Sheep, Xenopus, Zebrafish

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

16A11

Immunogen

Human HuC/HuD neuronal protein.

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Biotin
• Request custom conjugation

Form

Lyophilized

Purification

purified

Storage buffer

HEPES buffered saline

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_221448

Product Specific Information

This antibody recognizes the Elav family members HuC, HuD and Hel-N1 neuronal proteins. It does not recognize HuR, another Elav family member that is present in all proliferating cells. The antibody has been shown to specifically label neuronal cells in zebrafish, chick, canaries, and humans, and is likely to label neuronal cells in most vertebrate species. Labeling is visible early in development, at about the time that the neurons leave the mitotic cycle.

Storage and reconstitution: Upon receipt, lyophilized antibodies should be stored desiccated at -20ºC or lower. When properly stored, these products are stable for at least one year. To prepare stock solution, reconstitute the antibody in 0.5-1 mL PBS, pH 7.4, containing 1% BSA. Store the solution for up to two weeks at 4ºC with the addition of 2mM sodium azide. For longer storage, divide solutions into single-use aliquots and freeze at -20ºC, avoidig freeze/thaw cycles.

For best results in immunohistochemistry, antigen retrieval is required. Use 50mM Tris, pH 8.0, for 30 minutes.

A-21271 was successfully used to detect HuC/HuD in neurons differentiated from H9 ESC derived NSCs.

Target Information

Anti-Hu antibodies were originally isolated from a patient with paraneoplastic encephalomyelitis, a rare condition of selective neural tissue injury associated with small cell lung carcinoma. The antibodies bind specifically to antigens present exclusively in neuronal cells and are thus useful as markers of neuronal cells in tissue. The Hu antigen is an RNA-binding protein of the embryonic lethal abnormal visual (Elav) family.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.