Thermo Fisher Scientific HuC/HuD Monoclonal Antibody (16A11)

Thermo Fisher Scientific HuC/HuD Monoclonal Antibody (16A11)

Applications and Tested Dilution

Product Specifications

Additional Formats

• Biotin conjugate (A-21272)
• Request custom conjugation

Product Specific Information

This antibody recognizes the Elav family members HuC, HuD, and Hel-N1 neuronal proteins. It does not recognize HuR, another Elav family member present in proliferating cells. The antibody specifically labels neuronal cells in zebrafish, chick, canaries, and humans, and is likely to label neuronal cells in most vertebrate species. Labeling is visible early in development, when neurons exit the mitotic cycle.

Storage and Reconstitution:
Upon receipt, store lyophilized antibodies desiccated at -20°C or lower. Stable for at least one year when properly stored. Reconstitute in 0.5–1 mL PBS (pH 7.4) containing 1% BSA. Store at 4°C for up to two weeks with 2 mM sodium azide. For longer storage, aliquot and freeze at -20°C, avoiding freeze/thaw cycles.

Immunohistochemistry Recommendation:
For best results, antigen retrieval is required. Use 50 mM Tris (pH 8.0) for 30 minutes.

Experimental Note:
A-21271 was successfully used to detect HuC/HuD in neurons differentiated from H9 ESC-derived NSCs.

Target Information

Anti-Hu antibodies were originally isolated from a patient with paraneoplastic encephalomyelitis, a rare condition associated with small cell lung carcinoma. These antibodies bind specifically to antigens present exclusively in neuronal cells and serve as markers for neuronal tissue. The Hu antigen is an RNA-binding protein of the embryonic lethal abnormal visual (Elav) family.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.