Thermo Fisher Scientific RHBDD3 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:500

-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human RHBDD3. Recombinant protein control fragment (Product #RP-99855). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:RHBDD3,uniProtId:Q9Y3P4-1,ncbiNodeId:9606,antigenRange:249-327,antigenLength:386,antigenImageFileName:PA5-60571_RHBDD3_Q9Y3P4-1_Rabbit.svg,antigenImageFileNamePDP:PA5-60571_RHBDD3_Q9Y3P4-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2646526

Product Specific Information

Immunogen sequence: HWEDSALPPP SLRPVQPTWE GSSEAGLDWA GASFSPGTPM WAALDEQMLQ EGIQASLLDG PAQEPQSAPW LSKSSVSSL

Highest antigen sequence identity to the following orthologs: Mouse - 66%, Rat - 62%.

Target Information

The Rhomboid family of proteins is made up of several widely conserved polytopic membrane serine proteases that play roles in growth and development. RHBDD3 was initially identified as a novel pituitary tumor apoptosis gene (PTAG) whose expression was reduced in certain pituitary adenomas. Overexpression of RHBDD3 in AtT20 cells showed an increase in apoptotic activity and caspase activation in response to bromocriptine, and apoptosis-inducing dopamine D2 analog, suggesting that reactivation of RHBDD3 in tumors may be a therapeutically useful tool. Later experiments also showed that RHBDD3 expression is also reduced in several primary colorectal tumors, indicating that loss of RHBDD3 contributes to a blunted apoptotic response and probably predisposes cells towards malignant transformation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.