Thermo Fisher Scientific CD20 Monoclonal Antibody (2H7), NovaFluor Red 725, eBioscience

Applications

• Flow Cytometry (Flow)
  • Tested Dilution: 5 µL (0.4 µg)/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 532
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 496 / 615 / 737 / 805
• Brilliant Violet 421 / 605 / 650 / 711
• eFluor 450 / 506
• FITC
• NovaFluor Blue 610-70S / 660-120S
• NovaFluor Red 710
• NovaFluor Yellow 590 / 660
• PE / PE-Cyanine5 / PE-Cyanine5.5 / PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436 / 600 / 645 / 702 / 780
• Custom conjugation available

Product Specific Information

The 2H7 monoclonal antibody reacts with human CD20, a 33–36 kDa transmembrane protein expressed by developing and mature B cells, but not by plasma cells. Low-level expression may be found in a subset of mature T cells. CD20 is involved in B-cell activation.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested on normal human peripheral blood cells.
Recommended use: 5 µL (0.4 µg) per test in 100 µL total volume. Cell number: 10⁵–10⁸ cells/test.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes such as propidium iodide, 7-AAD, or DAPI.
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability analysis.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) must be used for optimal staining.
  • Add 5 µL per sample to antibody cocktails before mixing with cells.
  • For single-color controls, use 5 µL per 100 µL of cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology, based on nucleic acid dye structures engineered to minimize spectral spillover and spread.

Excitation: 636 nm
Emission: 727 nm
Laser: 633–640 nm (Red)

Target Information

CD20 is a non-glycosylated surface phosphoprotein (33–37 kDa) expressed on mature and most malignant B cells, some T lymphocytes, and follicular dendritic cells. It regulates calcium conductance, cell cycle progression, and B-cell proliferation. CD20 remains on the cell surface after receptor ligation, making it an effective target for antibody-mediated B-cell depletion.
Diseases associated with CD20 dysfunction include Ms4a1-related common variable immune deficiency.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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