Thermo Fisher Scientific UBE2B Recombinant Rabbit Monoclonal Antibody (2Z3R2)
UBE2B 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, ELISA에 사용 가능. HEK293 발현 시스템 기반, 높은 특이성과 재현성 제공. 인간, 생쥐, 랫드 반응성. 연구용으로만 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:6,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:5,000 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 2Z3R2 |
| Immunogen | Synthetic peptide corresponding to amino acids 53–152 of human UBE2B (UniProt ID: P63146) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.33 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2911741 |
Product Specific Information
Positive test controls include: HeLa, 293T, NIH/3T3, K-562, Mouse testis, Mouse heart, Rat testis.
The target is usually found in the following locations: Cell membrane, Nucleus.
Immunogen sequence:
FKLVIEFSEE YPNKPPTVRF LSKMFHPNVY ADGSICLDIL QNRWSPTYDV SSILTSIQSL LDEPNPNSPA NSQAAQLYQE NKREYEKRVS AIVEQSWNDS
Target Information
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin-protein ligases (E3s).
UBE2B is a member of the E2 ubiquitin-conjugating enzyme family and is required for post-replicative DNA damage repair.
Its protein sequence is 100% identical to mouse, rat, and rabbit homologs, indicating high conservation in eukaryotic evolution.
For Research Use Only. Not for use in diagnostic procedures or resale without express authorization.
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