Thermo Fisher Scientific Nkx2.6 Polyclonal Antibody
Human NKX2-6 단백질을 인식하는 Rabbit Polyclonal Antibody로 Western Blot 및 Immunocytochemistry에 적합. 합성 펩타이드 유래 면역원 사용. 액상 형태, 1 mg/mL 농도, -20°C 보관. 연구용으로만 사용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:1,000
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:100–1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human NKX2-6 (Accession A6NCS4), corresponding to amino acid residues G224–A274 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2851076 |
Product Specific Information
Antibody detects endogenous levels of total NKX2-6.
Target Information
Members of the NK-2 family of homeodomain proteins are key regulators of growth and development in several tissues, including brain, heart, and pancreas. Nkx-2.5, also designated cardiac-specific homeobox protein (Csx), is a homolog of the Drosophila tinman protein and is essential for normal cardiovascular development. Expression of Nkx-2.5 during cardiomyogenesis is required for cardiac septation, in which a single atrium and ventricle are separated into four chambers.
Nkx-2.5 binds to DNA as a monomer, a homodimer, or as a heterodimer with Nkx-2.3 or Nkx-2.6, which suggests that the specific protein-protein interactions of Nkx-2.5 are involved in its transcriptional regulatory function. Nkx-2.6, also a homolog of the Drosophila tinman protein, is expressed in the caudal pharyngeal pouches, caudal heart progenitors, sinus venosus, outflow tract of the heart, and a short segment of the gut between stages E8.5 and E10.5 of embryogenesis. Expression of Nkx-2.6 overlaps with that of Nkx-2.5 in the pharynx and heart. However, Nkx-2.6 mutant mice are viable and fertile, which suggests that Nkx-2.6 plays a compensatory function to Nkx-2.5.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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