Thermo Fisher Scientific DOT1L Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:10,000

-

Immunohistochemistry (IHC)

1:200-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

-

Immunoprecipitation (IP)

2-5 µg/mg lysate

-

ChIP-sequencing (ChIP-Seq)

4 µg/30 µg chromatin

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Region between residue1000 and 1050 of human DOT1-Like.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.20 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

TBS, pH 7.0 to 8.0, with 0.1% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Wet ice

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Application Note: For IHC, epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.

Target Information

Histone methyltransferase. Methylates Lys-79 of histone H3. Nucleosomes are preferred as substrate compared to free histones. Binds to DNA.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.