
Thermo Fisher Scientific CDKN1B Monoclonal Antibody (2F4)
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Applications
Tested Dilution
Publications
Western Blot (WB)
1-5 µg/mL
ELISA (ELISA)
10 µg/mL
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse / IgG2b, kappa
Class
Monoclonal
Type
Antibody
Clone
2F4
Immunogen
CDKN1B (ENSP00000228872, 100 a.a. approximately 198 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. if (typeof window.$mangular === undefined
|| !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{
targetFamily:
p27Kip1,
uniProtId:
P46527-1,
ncbiNodeId:
9606,
antigenRange:
100-198,
antigenLength:
198,
antigenImageFileName:
H00001027-M02_p27Kip1_P46527-1_House_mouse.svg,
antigenImageFileNamePDP:
H00001027-M02_p27Kip1_P46527-1_House_mouse_PDP.jpeg,
sortOrder:
1}\]
; $mangular.isB2BCMGT = false
; $mangular.isEpitopesModalImageMultiSizeEnabled = true
;
View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}
Conjugate
Unconjugated Unconjugated Unconjugated
Form
Liquid
Concentration
See Label
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Sequence of this protein is as follows: KVPAQESQDV SGSRPAAPLI GAPANSEDTH LVDPKTDPSD SQTGLAEQCA GIRKRPATDD SSTQNKRANR TEENVSDGSP NAGSVEQTPK KPGLRRRQT
Target Information
p27 Kip1 functions as a negative regulator of G1 mouse expression and has been proposed to function as a possible mediator of TGFb induced G1 arrest. p27 Kip1 is an important regulator of cell cycle progression, a potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. p27 Kip1 forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. p27 Kip1 acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry. p27 Kip1 has a molecular weight of approximately 27kDa and the degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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