Thermo Fisher Scientific Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 555

Applications

Tested Dilution

Western Blot (WB)

0.1-0.4 µg/mL

Immunocytochemistry (ICC/IF)

1-10 µg/mL

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Gamma Immunoglobins Heavy and Light chains

Conjugate

Alexa Fluor™ Plus 555 Alexa Fluor™ Plus 555 Alexa Fluor™ Plus 555

View additional formats

• [Alexa Fluor 350 ](/antibody/secondary/product-name/Mouse IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor 350/host/Goat/)
• [Alexa Fluor 488 ](/antibody/secondary/product-name/Mouse IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor 488/host/Goat/)
• [Alexa Fluor 546 ](/antibody/secondary/product-name/Mouse IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor 546/host/Goat/)
• [Alexa Fluor 555 ](/antibody/secondary/product-name/Mouse IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor 555/host/Goat/)
• [Alexa Fluor 568 ](/antibody/secondary/product-name/Mouse IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor 568/host/Goat/)
• [Alexa Fluor 594 ](/antibody/secondary/product-name/Mouse IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor 594/host/Goat/)
• [Alexa Fluor 633 ](/antibody/secondary/product-name/Mouse IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor 633/host/Goat/)
• [Alexa Fluor 635 ](/antibody/secondary/product-name/Mouse IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor 635/host/Goat/)
• [Alexa Fluor 647 ](/antibody/secondary/product-name/Mouse IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor 647/host/Goat/)
• [Alexa Fluor 660 ](/antibody/secondary/product-name/Mouse IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor 660/host/Goat/)
• [Alexa Fluor 680 ](/antibody/secondary/product-name/Mouse IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor 680/host/Goat/)
• [Alexa Fluor 790 ](/antibody/secondary/product-name/Mouse IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor 790/host/Goat/)
• [Alexa Fluor Plus 405 ](/antibody/secondary/product-name/Mouse IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor Plus 405/host/Goat/)
• [Alexa Fluor Plus 488 ](/antibody/secondary/product-name/Mouse IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor Plus 488/host/Goat/)
• [Alexa Fluor Plus 594 ](/antibody/secondary/product-name/Mouse IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor Plus 594/host/Goat/)
• [Alexa Fluor Plus 647 ](/antibody/secondary/product-name/Mouse IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor Plus 647/host/Goat/)
• [Alexa Fluor Plus 680 ](/antibody/secondary/product-name/Mouse IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor Plus 680/host/Goat/)
• [Alexa Fluor Plus 800 ](/antibody/secondary/product-name/Mouse IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor Plus 800/host/Goat/)
• [AP](/antibody/secondary/product-name/Mouse IgG (H--L) Highly Cross-Adsorbed/conjugates/AP/host/Goat/)
• [Biotin ](/antibody/secondary/product-name/Mouse IgG (H--L) Highly Cross-Adsorbed/conjugates/Biotin/host/Goat/)
• [DSB-X Biotin](/antibody/secondary/product-name/Mouse IgG (H--L) Highly Cross-Adsorbed/conjugates/DSB-X Biotin/host/Goat/)
• [FITC](/antibody/secondary/product-name/Mouse IgG (H--L) Highly Cross-Adsorbed/conjugates/FITC/host/Goat/)
• [HRP](/antibody/secondary/product-name/Mouse IgG (H--L) Highly Cross-Adsorbed/conjugates/HRP/host/Goat/)
• [TRITC](/antibody/secondary/product-name/Mouse IgG (H--L) Highly Cross-Adsorbed/conjugates/TRITC/host/Goat/)
• Request custom conjugation

Excitation/Emission Max

558/572 nm View spectra

Form

Liquid

Concentration

2 mg/mL

Purification

Affinity chromatography

Storage buffer

proprietary buffer, pH 6.5

Contains

0.016% Methylisothiazolone, 0.016% Bromonitrodioxane

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2633276

Target

IgG

Cross Adsorption

Against bovine IgG, goat IgG, rabbit IgG, rat IgG, human IgG and human serum

Antibody Form

Whole Antibody

Product Specific Information

To minimize cross-reactivity, the goat anti-mouse IgG whole antibodies have been pre cross-adsorbed against bovine IgG, goat IgG, rabbit IgG, rat IgG, human IgG, and human serum. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in less background staining and cross-reactivity. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. Further passages through additional columns result in highly cross-adsorbed preparations of secondary antibody. The benefits of these extra steps are apparent in multiplexing/multicolor-staining experiments where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins.

Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically.

Target Information

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.