Thermo Fisher Scientific Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 555

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Alexa Fluor Plus 555 결합 염소 유래 항-마우스 IgG(H+L) 2차 항체로, 고도로 교차흡착되어 비특이적 반응을 최소화합니다. Western blot 및 면역형광에 적합하며, 높은 신호 대 잡음비와 우수한 형광 안정성을 제공합니다. 연구용으로만 사용 가능합니다.

카탈로그번호: A32727
브랜드: Thermo Fisher Scientific
카테고리: Polyclonal Antibodies
판매단위: pk

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마지막 업데이트 2025. 08. 04. 오전 11:13

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Thermo Fisher Scientific A32727 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 555 1 mg pk판매 단위 pk · 1~2일 내 출고

재고 1개재고 1개1~2일 내 출고

550,400원VAT 포함 605,440원

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Thermo Fisher Scientific · Thermo Fisher Scientific Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 555

Applications and Tested Dilution

Application	Tested Dilution
Western Blot (WB)	0.1–0.4 µg/mL
Immunocytochemistry (ICC/IF)	1–10 µg/mL

Product Specifications

항목	내용
Species Reactivity	Mouse
Host / Isotype	Goat / IgG
Class	Polyclonal
Type	Secondary Antibody
Immunogen	Gamma Immunoglobins Heavy and Light chains
Conjugate	Alexa Fluor™ Plus 555
Excitation / Emission Max	558 / 572 nm
Form	Liquid
Concentration	2 mg/mL
Purification	Affinity chromatography
Storage Buffer	Proprietary buffer, pH 6.5
Contains	0.016% Methylisothiazolone, 0.016% Bromonitrodioxane
Storage Conditions	4°C, store in dark
Shipping Conditions	Ambient (domestic); Wet ice (international)
RRID	AB_2633276
Target	IgG
Cross Adsorption	Against bovine IgG, goat IgG, rabbit IgG, rat IgG, human IgG and human serum
Antibody Form	Whole Antibody

Product Specific Information

To minimize cross-reactivity, the goat anti-mouse IgG whole antibodies have been pre cross-adsorbed against bovine, goat, rabbit, rat, and human immunoglobulins and serum. This cross-adsorption purification step enhances specificity, reducing background staining and cross-reactivity.
The secondary antibody solution is passed through column matrices containing immobilized serum proteins from potentially cross-reactive species. Only nonspecific-binding antibodies are captured, while highly specific antibodies flow through. Additional purification steps yield highly cross-adsorbed antibody preparations.

These extra purification steps are particularly beneficial in multiplex or multicolor staining experiments, or in tissues containing endogenous immunoglobulins.

Usage Note:
Centrifuge the conjugate solution briefly before use and use only the supernatant to remove potential aggregates. Optimal dilution should be determined empirically for each application.

Target Information

Anti-Mouse secondary antibodies are affinity-purified with high specificity for mouse immunoglobulins. They are useful for detection, sorting, or purification of target proteins.
Secondary antibodies provide versatility across multiple detection systems (e.g., HRP, AP, fluorescence) and greater sensitivity via signal amplification. They are typically generated by immunizing the host with pooled mouse immunoglobulins and further purified or modified (e.g., immunoaffinity chromatography, fragmentation, label conjugation) to yield highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.