Thermo Fisher Scientific SPIN2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human SPIN2. Recombinant protein control fragment (Product #RP-91960). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SPIN2,uniProtId:Q9BPZ2-1,ncbiNodeId:9606,antigenRange:132-238,antigenLength:258,antigenImageFileName:PA5-51357_SPIN2_Q9BPZ2-1_Rabbit.svg,antigenImageFileNamePDP:PA5-51357_SPIN2_Q9BPZ2-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2647854

Product Specific Information

Immunogen sequence: AVEHMFEGEH GSKDEWRGMV LAQAPIMKAW FYITYEKDPV LYMYQLLDDY KEGDLRIMPE SSESPPTERE PGGVVDGLIG KHVEYTKEDG SKRIGMVIHQ VEAKPSV

Highest antigen sequence identity to the following orthologs: Mouse - 92%, Rat - 93%.

Target Information

Human SPIN2 expressed by 2 IL3-dependent murine myeloid cell lines was moderately antiapoptotic following IL3 removal, but it was unable to block apoptosis induced by the chemotherapy agent doxorubicin. On the contrary, SPIN2-expressing cells were more sensitive to doxorubicin-mediated cell death. Also, SPIN2 did not combat the proapoptotic effects of Fas ligand stimulation in the Jurkat human T-cell line. Deletion of the C-terminal amino acids of SPIN2 diminished its antiapoptotic activity. In the murine myeloid cells, SPIN2 overexpression increased the cell number doubling times and slowed their rate of growth. There was also an increased percentage of cells in G2/M, which was more pronounced following IL3 withdrawal.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.