Thermo Fisher Scientific IkB beta Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human NFKBIB. Recombinant protein control fragment (Product #RP-105278). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:IkB beta,uniProtId:Q15653-1,ncbiNodeId:9606,antigenRange:193-257,antigenLength:356,antigenImageFileName:PA5-84615_IkB_beta_Q15653-1_Rabbit.svg,antigenImageFileNamePDP:PA5-84615_IkB_beta_Q15653-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.4 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2791766

Product Specific Information

Immunogen sequence: EEDWKLQLEA ENYEGHTPLH VAVIHKDVEM VRLLRDAGAD LDKPEPTCGR SPLHLAVEAQ AADVL

Target Information

The NF-kB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IkB proteins. Most agents that activate NF-kB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IkB. The key regulatory step in this pathway involves activation of a high molecular weight IkB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKK alpha and IKK beta serve as the catalytic subunits of the kinase and IKK gamma serves as the regulatory subunit. Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKK beta (Ser176 and Ser180 in IKK alpha), which causes conformational changes, resulting in kinase activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.