Thermo Scientific™

SuperSignal™ West Pico PLUS Chemiluminescent Substrate

Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram자세히 알아보기

Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram to high femtogram-level protein detection by western blot analysis.

SuperSignal West Pico PLUS Chemiluminescent Substrate characteristics include:

• Sensitivity: pictogram to femtogram sensitivity
• Stability: 8-hr working solution stability; 1-year kit stability at room temperature
• Compatibility: nitrocellulose and PVDF membranes
• Signal duration: 6 to 24 hours
• Recommended primary antibody concentration: 1:1000–1:5000 dilution (0.2–1.0 μg/mL)
• Recommended secondary antibody concentration: 1:20,000–100,000 dilution (10–50 ng/mL)

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SuperSignal West Pico PLUS substrate is designed to provide excellent signal intensity and sensitivity for western blotting with HRP conjugates. The intensity of the light emission combined with the exceptional duration allows for the acquisition of multiple exposures to more easily obtain publication-quality blot images. SuperSignal West Pico PLUS substrate is compatible with different membranes, blocking reagents, and a wide range of antibody dilutions, making it an ideal choice for most western blotting applications.

사양

제품 유형Substrate

검출 방법Chemiluminescence

형태Liquid

용도(애플리케이션)Western Blot

Membrane CompatibilityNitrocellulose, PVDF

제품라인SuperSignal™

수량200 mL

콘주게이트HRP

SubstrateHorseradish Peroxidase

권장 항체 농도2°: 1:20-100 K (10-50 ng/mL), 1°: 1:1 K (0.2-1 μg/mL)

민감도Picogram to high femtogram-level

신호 지속 시간6 to 24 hr.

충분25 Mini-Gel Blots, 2000 cm2 of Membrane

키트 구성품SuperSignal West Pico PLUS Luminol/Enhancer, SuperSignal West Pico PLUS Stable Peroxide

배송 조건Room Temperature

Unit SizeEach