Thermo Fisher Scientific ZAP70 (Chronic Lymphocytic Leukemia Marker) Monoclonal Antibody (ZAP70/528, 2F3.2)
인체 ZAP70 단백질을 인식하는 단클론 항체로, 만성 림프구성 백혈병(CLL) 마커 연구에 적합. IHC(P) 및 IHC(PFA) 응용 가능. 고순도 Protein A/G 정제, 1 mg/mL 농도. PBS(pH 7.4)에 보존제 없이 액상 형태로 제공.
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Applications
Immunohistochemistry (Paraffin) (IHC (P))
- Assay-dependent
Immunohistochemistry (PFA fixed) (IHC (PFA))
- 1–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | ZAP70/528, 2F3.2 |
| Immunogen | Recombinant full-length human ZAP70 protein (ZAP70/528). Recombinant ZAP-70 protein including residues 1–254 encompassing SH2 domains of human ZAP70 (2F3.2). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
ZAP-70 is a cytosolic protein tyrosine kinase belonging to the Syk family. It is expressed exclusively in T-cells and natural killer cells and is essential for T-cell receptor activation. Upon T-cell antigen receptor (TCR) engagement, ZAP70 is phosphorylated at tyrosines 292, 315, and 319 in the interdomain B region between SH2 and kinase domains. Phosphorylation at tyrosines 315 (Vav binding site) and 319 (Lck binding site) enhances ZAP70-mediated lymphocyte signaling, whereas tyrosine 292 phosphorylation attenuates activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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