Thermo Fisher Scientific Pierce Polyacrylamide Desalting Columns, 1.8K MWCO, 5 mL

Thermo Scientific™ Pierce™ Polyacrylamide Desalting Columns, 1.8K MWCO, 5 mL

Pierce™ 1.8K MWCO Polyacrylamide Desalting Columns are pre-packed with porous polyacrylamide beads and provide efficient buffer exchange and desalting of proteins and other macromolecular samples based on a molecular-weight cutoff (MWCO) of 1800.

Unlike other size-exclusion media, polyacrylamide resin is not subject to enzymatic degradation and does not serve as a nutrient for microbial growth. The resin is highly hydrophilic, minimizing undesirable binding interactions between resin and sample molecules. Contamination with low molecular weight sugars is not a concern when using polyacrylamide resin.

Oxidizing agents can be removed without damaging the support. The resin is susceptible to hydrolysis under extreme pH conditions; therefore, an operating pH range of 2–10 is recommended at room temperature. It can be autoclaved at pH 5.5–6.5 for 30 minutes at 120°C.

These columns use resin beads with a wet diameter of 45–90 μm and are ideal for separating peptides and small macromolecules (>1.8 kDa) from buffer salts and other low molecular weight compounds (<500 MW).

Features of Polyacrylamide Resin

• Stable in water, salt solutions, organic solvents, and acidic or alkaline conditions
• Excellent flow properties
• Heat-stable

Applications

• Removing salts from protein solutions
• Removing phenol from nucleic acid preparations
• Separating excess crosslinker from conjugate preparations
• Removing excess derivatizing agents from modified proteins
• Removing unreacted dye from fluorescent antibodies
• Removing free radiolabel from labeled proteins
• Exchanging one buffer for another

Principle of Operation

Gravity-flow resin filtration separates molecules based on size and ability to penetrate the stationary phase. The chromatographic matrix consists of small, uncharged porous particles packed into a column. Large molecules are excluded from the pores and elute first, while smaller molecules penetrate the pores and elute later with additional buffer volume.
Desalting and buffer exchange are among the most common applications of resin filtration chromatography.

Related Products

• Pierce™ Polyacrylamide Spin Desalting Columns, 7K MWCO, 0.7 mL
• Polyacrylamide Desalting Columns, 6K MWCO, 10 mL

제품 사양