Ion Torrent™

Oncomine™ Myeloid MRD RNA Assay (RUO)

The Ion Torrent Oncomine Myeloid MRD RNA Assay (RUO) is a comprehensive targeted next-generation sequencing (NGS) assay designed for myeloid자세히 알아보기

The Ion Torrent Oncomine Myeloid MRD RNA Assay (RUO) is a comprehensive targeted next-generation sequencing (NGS) assay designed for myeloid measurable residual disease (MRD) research. It is an RNA-only version of the Oncomine Myeloid MRD Assay (Cat. No. A53837). IonAmpliSeq HD technology enables variant detection with sensitivity as low as 0.05% allele frequency.

The panel includes over 900 key RNA fusions for myeloid MRD analysis. These carefully curated targets are relevant for all categories of myeloid neoplasms, including acute myeloid leukemia (AML), myelodysplastic syndrome (MDS), and myeloproliferative neoplasm (MPN) samples.

This assay is compatible with the Ion GeneStudio S5 sequencer and can be run on either Ion 540 or Ion 550 chips. The input material is RNA extracted from blood and bone marrow samples. The test includes a complete informatics and reporting solution that simplifies data analysis and interpretation.

Oncomine Myeloid MRD RNA Assay features include:
• Comprehensive coverage of key RNA fusions associated with myeloid disorders
• Effective designs for key fusions targets such as BCR-ABL1, RUNX1, and KMT2A
• Fast 2–3 day sample-to-report workflow
• Analytically validated end-to-end informatics and reporting solution

The Oncomine Myeloid MRD RNA Assay is designed to assist in myeloid MRD research. The RNA panel includes a broad fusion panel of 27 driver genes, with the potential to detect >900 different fusion isoforms.

The multiplex PCR primer design leverages Ion AmpliSeq HD technology to generate results from multiple barcoded samples in a single run and dual barcoding ensures high sensitivity and accuracy. Libraries are prepared manually using a simple one-day PCR-based workflow and quantified by qPCR. Chip templating is done on the Ion Chef instrument overnight, followed by a ∼3–4 hr sequencing run on the Ion GeneStudio S5 system. Sequencing data is automatically analyzed by Ion Reporter software using optimized assay-specific analysis workflows and can be annotated and exported into customizable reports.

The assay kit includes one pool of Ion AmpliSeq HD oligonucleotide RNA primers and Ion AmpliSeq HD library preparation reagents sufficient to sequence up to 24 samples. Twelve RNA samples can be sequenced together on an Ion 540 chip, and eighteen RNA samples can be sequenced together on an Ion 550 chip. Ion GeneStudio sequencing chips (Ion 540 or Ion 550) and sequencing reagents must be ordered separately.

사양

칩 호환성540 and 550 chips

형식Kit

샘플 종류RNA from Blood or Bone Marrow

Sequencing TypeNext Generation Sequencing

용도(장비)Ion GeneStudio S5 Sequencer

수량24 Reactions

Unit SizeEach