Thermo Fisher Scientific Synaptotagmin 1 Polyclonal Antibody
Rabbit polyclonal antibody against human Synaptotagmin 1. Suitable for WB and IHC at 1:300–1:2,000 dilution. Recognizes SYTI with high specificity. Lyophilized form, reconstitute in sterile water. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:300–1:2,000 | – |
| Immunohistochemistry (IHC) | 1:300–1:2,000 | – |
| Miscellaneous (PubMed) | – | View 1 publication |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / Ig |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from human Synaptotagmin 1 conjugated to blue carrier protein |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not Determined |
| Storage Buffer | Whole serum |
| Contains | No preservative |
| Storage Conditions | Store at 4 °C short term. For long-term storage, store at −20 °C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Specificity: SYTI.
Target Information
Synaptotagmin 1 is a synaptic vesicle membrane glycoprotein widely expressed throughout the CNS. It functions as a Ca²⁺ sensor regulating exocytosis and neurotransmitter release. Synaptotagmin’s Ca²⁺-mediated binding of SNAP25 is essential for Ca²⁺-dependent membrane fusion. Specific residues within the C2B domain influence endocytic rate and vesicle size. Synaptotagmin 1 regulates membrane interactions during synaptic vesicle trafficking and binds acidic phospholipids requiring both an acidic head group and diacyl backbone. It interacts with receptors for activated protein kinase C in a Ca²⁺-dependent manner and with neurexins, syntaxin, and AP2 in a Ca²⁺-independent manner.
Dysfunction of Synaptotagmin 1 is associated with food-borne and infant botulism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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