Thermo Fisher Scientific SHIP1 Recombinant Rabbit Monoclonal Antibody (2F8U0)
SHIP1 단백질을 인식하는 재조합 토끼 단클론 항체로, WB, IHC, ICC/IF, ELISA 등에 사용 가능. 인간, 생쥐, 랫트 반응성. HEK293 발현 시스템에서 제조되었으며, 액상 형태로 제공. 세포막 및 세포질 내 SHIP1 단백질 검출에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 2F8U0 |
| Immunogen | Recombinant fusion protein containing amino acids 1084–1178 of human SHIP1 (UniProt ID: Q92835) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.75 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 0.05% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2911964 |
Product Specific Information
- Positive test controls: Raji, U-937
- Localization: Cytoplasm, Membrane, Peripheral membrane protein
Immunogen sequence:
RSFTCSSSAE GRAAGGDKSQ GKPKTPVSSQ APVPAKRPIK PSRSEINQQT PPTPTPRPPL PVKSPAVLHL QHSKGRDYRD NTELPHHGKH RPEEG
Target Information
This gene belongs to the inositol polyphosphate-5-phosphatase (INPP5) family. It encodes a protein with an N-terminal SH2 domain, an inositol phosphatase domain, and two C-terminal protein interaction domains. Expression is restricted to hematopoietic cells, where movement from the cytosol to the plasma membrane is mediated by tyrosine phosphorylation. At the plasma membrane, the protein hydrolyzes the 5' phosphate from phosphatidylinositol (3,4,5)-trisphosphate and inositol-1,3,4,5-tetrakisphosphate, affecting multiple signaling pathways. It functions as a negative regulator of myeloid cell proliferation and survival. Alternate splice variants produce different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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