Thermo Scientific™

DTBP (Wang and Richard`s Reagent)

Thermo Scientific Pierce DTBP is dimethyl 3,3`-dithiobispropionimidate, a crosslinker that contains amine-reactive imidoester groups around an 8-atom spacer arm, whose자세히 알아보기

Thermo Scientific Pierce DTBP is dimethyl 3,3`-dithiobispropionimidate, a crosslinker that contains amine-reactive imidoester groups around an 8-atom spacer arm, whose central disulfide bond can be cleaved with reducing agents.

Dimethyl dithiobispropionimidate (DTBP) is a homobifunctional, cleavable and membrane permeable crosslinker. It contains an amine-reactive imidoester at each end of an 8-atom spacer arm. Imidoesters react with primary amines at pH 8-10 to form stable amidine bonds. The amidine is protonated and therefore, like the lysine it modified, has a positive charge at physiological pH. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for imidoester crosslinking reagents.

Features of dimethyl 3,3`-dithiobispropionimidate:

• Reactive groups: imidoester (both ends)
• Reactive towards: amino groups (primary amines)
• Imidoester reacts rapidly with any primary amine-containing molecule to form amidine bonds
• Cleavable by reduction of spacer arm disulfide bond with DTT, TCEP or other reducing agents
• Compare to DMS (non-cleavable)
• Water-soluble

Applications:
• Reversibly crosslink and stabilize protein-protein interactions
• Stabilize protein interactions prior to ChIP assays to capture indirect protein interactions

DTBP crosslinks can be easily reversed by the addition of 100-150 mM DTT at 37°C for 30 minutes. DMS, the non-cleavable analog of DTBP is available for applications that require a stable, non-cleavable bond. DTSSP, the NHS-ester analog of DTBP is also available for applications that require crosslinking at physiologic pH or greater stability of the crosslinked product at elevated pH. DTBP and DMS have essentially identical crosslinking activity toward primary amines.

Properties of DTBP
• Alternate names: Cleavable DMS
• Molecular weight: 309.28
• Spacer arm length: 11.9 Å (8 atoms)
• CAS number: 38285-78-8
• Reactive groups: Imidoesters, react with primary amines at pH 8–10

DTBP Specifications
We manufacture DTBP to the highest specifications to produce the most specific bioconjugates, ensure the integrity of your data and to provide you with the highest degree of consistency. Each lot of DTBP cross-linker is tested to meet the following minimum specifications:
• Identity: IR scan shows only peaks characteristic of the structure and functional groups of DTBP
• Melting point: begins melting between 170-178°C, melts within a 2°C range

Product References:
Crosslinker Application Guide -- search for recent literature references for this product

Related Products
DMA (dimethyl adipimidate)
DMP (dimethyl pimelimidate)
DMS (dimethyl suberimidate)

사양

화학물질 반응성Amine-Amine

CleavableBy Thiols

설명DTBP

분자량309.28

페길레이티드No

스페이서 암 길이11.9 Å

세포 투과성Yes

배송 조건Ambient

제품라인Pierce™

라벨링 방법Chemical Labeling

교차결합제 유형Homobifunctional

반응성 부분Imidoester

스페이서Medium (10 to 30 Å)

형태Powder

수량1 g

용해도Water

형식Standard

수용성Yes

제품 유형Crosslinker

Unit SizeEach