Thermo Fisher Scientific PER2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

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ELISA (ELISA)

1 µg/mL

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ChIP assay (ChIP)

5 µg antibody for 10 µg-15 µg of Chromatin

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Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 390-565 of human PER2 (NP_0737281) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PER2,uniProtId:O15055-1,ncbiNodeId:9606,antigenRange:390-565,antigenLength:1255,antigenImageFileName:PA5-89045_PER2_O15055-1_Rabbit.svg,antigenImageFileNamePDP:PA5-89045_PER2_O15055-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.88 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2805317

Product Specific Information

Immunogen sequence: GQPFDYSPIR FRARNGEYIT LDTSWSSFIN PWSRKISFII GRHKVRVGPL NEDVFAAHPC TEEKALHPSI QELTEQIHRL LLQPVPHSGS SGYGSLGSNG SHEHLMSQTS SSDSNGHEDS RRRRAEICKN GNKTKNRSHY SHESGEQKKK SVTEMQTNPP AEKKAVPAME KDSLGV

Positive Samples: Mouse liver; Cellular Location: Cytoplasm, Nucleus, nucleolus, perinuclear region

Target Information

PER2, also known as Period circadian protein homolog 2, is a mainly nuclear protein that shows nucleocytoplasmic shuttling which is effected by interaction with other circadian core oscillator proteins and/or by phosphorylation. PER2 belongs to the basic helix-loop-helix family of transcription factors and contains a PAC (PAS-associated C-terminal) domain and two PAS (PER-ARNT-SIM) domains. PER2 is a component of the circadian core oscillator, which includes the CRY proteins, CLOCK or NPAS2, BMAL1 or BMAL2, CSNK1D and/or CSNK1E, TIMELESS, and the PER proteins. This protein is thus essential for generating circadian rhythms and is a negative element in the circadian transcriptional loop which influences clock function by interacting with other circadian regulatory proteins and transporting them to the nucleus. Expression of PER2 is fairly wide spread with strong expression in skeletal muscle and pancreas and slight expression in lung. Defects in PER2 are a cause of familial advanced sleep-phase syndrome (FASPS).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.